Torrent Browser Analysis Report Guide


Classic Barcode Reports

This pages describes the old style run reports used before release 3.0. The old style run reports use an older layout and do not offer 4.x features. The Classic run report is viewed with the Classic Report button (near the top right of a run report page):

The Barcode Reports section displays histograms for the following metrics per barcode:

Chart

Description

Total number of reads

Total number of filtered and trimmed reads independent of length. This number is reported in the barcode unmapped BAM file.

AQ20 Bases

Number of base pairs of sequence from reads with aligned quality score of AQ20 or better.

Mean AQ20 read length

An AQ20 read length is the length, in bp units, that after alignment has a Phred-like score of 20 or better, or one error in 100 bp. This histogram charts the mean of these AQ20 read lengths per barcode.

AQ20 Reads

The number of reads with an aligned quality score of AQ20 or better.



The number of barcodes shown in the reports varies according to the barcode set used in your run and on the barcodes actually present in the sample. Only data for barcodes present in the run are displayed in the chart.

Each bar is labeled with the barcode ID. Data labeled as barcode ID X reports the number of unclassified barcodes: reads which could not be classified as matching one of the expected barcodes in the barcode set.