Torrent Browser Analysis Report Guide


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Analysis Report Guide TOC

Barcode Reports

The barcode section of a run report displays the following information per barcode:

Column

Description

Barcode Name

The individual barcode in the barcode set.

The row labeled as No barcode reports on unclassified barcodes, which are reads that could not be classified as matching one of the expected barcodes in the barcode set.

Sample Name of the sample that was sequenced on instrument.
Output Total number of reads.
% >= Q20

The percentage of reads that have a predicted quality score of Q20 or better.

A Q20 score is the predicted quality of a Phred-like score of 20 or better, or one error in 100 bp.

Reads

Total number of filtered and trimmed library reads (independent of length). This number is reported in the barcode BAM file.

Mean Read Length

The average read length, in bp, of all filtered and trimmed library reads reported in the barcode BAM file.

Read Length Histogram

A thumbnail histogram of the read lengths for this barcode. Click on the thumbnail histogram to open a larger image.

BAM

Buttons to download the BAM and BAM index file (BAI) for this barcode. The BAM file contains aligned reads sorted by reference location.

See also Run Metrics Overview for a description of alignment data.



The number of barcodes shown in the barcode section varies according to the barcode set used in your run and on the barcodes actually present in the sample. Only data for barcodes present in the run are displayed in the run report.