---

Torrent Suite Software space on Ion Community

Analysis Report Guide TOC

Torrent Variant Caller Integration with TaqMan and PCR

This page describes how to use your Torrent Variant Caller (TVC) results for pre-designed primers for PCR and Sanger sequencing or with the TaqMan Assay Search webpage .

Export to Pre-Designed Primers for PCR and Sanger Sequencing site

With this export option, you select variants to search for pre-designed primers for PCR and Sanger sequencing.

To initiate a search, use the checkboxes column (on the left) to select variants from the TVC plugin report and click on the Export Selected button. A pop-up window allows you to submit variants for the PCR and Sanger sequencing pre-designed primers site:

A new browser window opens to this site: Pre-Designed Primers for PCR and Sanger Sequencing . You must be connected to the internet to access this feature.

TaqMan Assay Search

After running the TVC plugin, you can select variants from a report table and submit a search against the TaqMan Assay Search webpage. When the search is submitted, you can choose which TaqMan Assay database to search. A new browser window appears with the search results. If TaqMan assays are available for the selected variant, you can immediately order the assay directly from the landing page. You must be connected to the internet to access this feature.

To initiate a search, use the checkboxes column (on the left) to select variants from the TVC plugin report and click the Export Selected button. A pop-up window allows you to submit variants for TaqMan assay design.

You are then presented with a list of verification assays that you can order from Life Technologies.

---