Torrent Browser Analysis Report Guide
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Example Torrent Variant Caller Parameter File
This page contains an example JSON-format parameters file.
A file of this type is used to upload custom parameter settings to the Torrent Variant Caller (TVC) plugin (see Upload a JSON file of parameter values ). You can download a similar file with the settings used in your TVC run, with the plugin report Settings File button (see Variant Caller Report ).
{ "torrent_variant_caller": { "snp_min_allele_freq": "0.15", "snp_strand_bias": "0.95", "hotspot_min_coverage": "20", "hotspot_min_cov_each_strand": "3", "hotspot_min_allele_freq": "0.15", "snp_min_variant_score": "10", "snp_beta_bias": "8", "hotspot_strand_bias": "0.95", "hp_max_length": "8", "filter_insertion_predictions": "0.2", "indel_min_variant_score": "10", "indel_beta_bias": "8", "indel_min_coverage": "20", "heavy_tailed": "3", "outlier_probability": "0.01", "data_quality_stringency": "8.5", "snp_min_cov_each_strand": "3", "hotspot_min_variant_score": "10", "indel_strand_bias": "0.85", "downsample_to_coverage": "400", "filter_unusual_predictions": "0.25", "indel_min_allele_freq": "0.15", "prediction_precision": "1", "indel_min_cov_each_strand": "3", "filter_deletion_predictions": "0.2", "snp_min_coverage": "20", "hotspot_beta_bias": "8" }, "meta": { "tvcargs": "tvc" }, "long_indel_assembler": { "short_suffix_match": "5", "min_indel_size": "4", "min_var_count": "5", "min_var_freq": "0.15", "kmer_len": "19", "max_hp_length": "8", "relative_strand_bias": "0.8" }, "freebayes": { "gen_min_coverage": "6", "allow_mnps": "0", "read_max_mismatch_fraction": "1", "allow_snps": "1", "allow_indels": "1", "min_mapping_qv": "4", "gen_min_alt_allele_freq": "0.15", "read_mismatch_limit": "10", "min_base_qv": "4", "gen_min_indel_alt_allele_freq": "0.15" } }
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See The Ion Reporter™ Software Integration Guide
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Torrent Variant Caller Plugin
Torrent Variant Caller Parameters
Example Torrent Variant Caller Parameter File
Torrent Variant Caller Output
The Command-Line Torrent Variant Caller
Ion Reporter™ Software Features Related to Variant Calling
Integration with TaqMan® and PCR