Torrent Browser Analysis Report Guide


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Analysis Report Guide TOC

Test Fragment Report

The Test Fragment Summary section of the Analysis Reportprovides information about the performance of each Test Fragment included in the experiment.

Test Fragments are used during analysis to predict the CF/IE/DR values for each Test Fragment, regionally.Analysis results for a Test Fragmentare displayed when there are at least 1000 high-quality Test Fragments, where there is an 85% match against the appropriate template in the Test Fragmentlist. This includes CF/IE/DR estimates and performance calculations.

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The number of TFs reported includes lower quality TFs, down to 70% match, to better represent the run quality from all TF's.

Open the test fragment report

Open the test fragments report with the Test Fragments button, near the bottom of the run report:

Test fragment metrics

The Test Fragments report displays the following information:

Parameter

Description

Test Fragment

Test fragment name (defined in the Admin > References tab of Torrent Browser).

Reads

Number of filtered & trimmed reads identified for this test fragment.

Percentage 50AQ17

The percentage of reads for this test fragment with a minimum of 50 base pairs in length and an error rate of 1 in 50, Phred-like 17, or better. Quality is based on alignment, not predicted quality.

The t est fragment sequence is also shown in the read length histogram.

Read length histogram

This is a histogram of read lengths, in bp units, that have a Phred-like score of 17 or better, or one error in 50 bp (the ends only are shown because of width considerations):

Distributions skewed to the right are ideal, showing longer read lengths (test fragments are a discrete length).It is likely that the sequence can extend all the way through the test fragment , if enough flows are run, so the histogram only displays a maximum size based on the length of the test fragment .