Torrent Browser Analysis Report Guide
Torrent Suite Software space on Ion Community
Test Fragment Report
The Test Fragment Summary section of the Analysis Reportprovides information about the performance of each Test Fragment included in the experiment.
Test Fragments are used during analysis to predict the CF/IE/DR values for each Test Fragment, regionally.Analysis results for a Test Fragmentare displayed when there are at least 1000 high-quality Test Fragments, where there is an 85% match against the appropriate template in the Test Fragmentlist. This includes CF/IE/DR estimates and performance calculations.
Open the test fragment report
Open the test fragments report with the Test Fragments button, near the bottom of the run report:
Test fragment metrics
The Test Fragments report displays the following information:
Parameter |
Description |
---|---|
Test Fragment |
Test fragment name (defined in the Admin > References tab of Torrent Browser). |
Reads |
Number of filtered & trimmed reads identified for this test fragment. |
Percentage 50AQ17 |
The percentage of reads for this test fragment with a minimum of 50 base pairs in length and an error rate of 1 in 50, Phred-like 17, or better. Quality is based on alignment, not predicted quality. |
The t est fragment sequence is also shown in the read length histogram.
Read length histogram
This is a histogram of read lengths, in bp units, that have a Phred-like score of 17 or better, or one error in 50 bp (the ends only are shown because of width considerations):
Distributions skewed to the right are ideal, showing longer read lengths (test fragments are a discrete length).It is likely that the sequence can extend all the way through the test fragment , if enough flows are run, so the histogram only displays a maximum size based on the length of the test fragment .
Torrent Browser Analysis Report Guide
Run Report Metrics
Run Metrics Overview
Run Report Metrics Before Alignment
Run Report Metrics on Aligned Reads
Barcode Reports
Test Fragment Report
Report Information
Output Files
Plugin Summary
Assembler SPAdes Plugin
Coverage Analysis Plugin
ERCC Analysis Plugin
FileExporter Plugin
FilterDuplicates Plugin
IonReporterUploader Plugin
See The Ion Reporter™ Software Integration Guide
Run RecognitION Plugin
SampleID Plugin
TorrentSuiteCloud Plugin
Torrent Variant Caller Plugin
Torrent Variant Caller Parameters
Example Torrent Variant Caller Parameter File
Torrent Variant Caller Output
The Command-Line Torrent Variant Caller
Ion Reporter™ Software Features Related to Variant Calling
Integration with TaqMan® and PCR