Torrent Browser Analysis Report Guide

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Torrent Suite Software space on Ion Community

Analysis Report Guide TOC

The SampleIDPlugin

Ion AmpliSeq Sample ID Panel is a human SNP genotyping panel enabling accurate sample verification for increased confidence in sample data management. The plugin is comprised of nine primer pairs that can be combined with any Ion AmpliSeq Ready-to-Use or Custom Panel for the generation of a unique ID during post-sequencing analysis of research samples.

The Ion AmpliSeq Sample ID Panel can be used in combination with any Ion AmpliSeq Ready-to-Use or Custom Panel using the Ion AmpliSeq Designer 1.2 or greater. This plugin is compatible with the Ion Xpress barcodes set.

Plugin output

Run the SampleID plugin automatically

Run the SampleID plugin manually

On-target metrics

Plugin output

Example plugin output is shown below:

Use the Sample ID column to verify sample fingerprints.

Click on a barcode ID to open the detail report:

With the detail report, you can review the IUPAC SNP calls. The contents of the TaqMan Assay ID column are links to order the corresponding TaqMan Assay.

Plugin configuration

The sampleID plugin does not take any options or configuration.

Run the SampleID plugin automatically

You set up the plugin to run automatically when you configure your template. In the Plugin chevron of the template wizard, you select which plugins run automatically on planned runs created from that template. See the Templates page in the Torrent Browser User Interface Guide .

Run the SampleID plugin manually

You can launch the plugin manually from a completed run report.

Follow these steps to run the plugin manually:

1. Open the run report and scroll down to the Plugin Summary button. Click Select plugins to run .
   
   
   
   
   
   
   
   
2. In the Select a plugin list, click sampleID . The sampleID plugin does not take user input. The plugin executes immediately (depending on server load) when you click it in the Select a plugin list.

On-target metrics

When you use the SampleID Panel, lower-than-expected number of on-target reads may occur.To recover the correct on-target reads metrics, add back the On-Target reads from the Sample ID Panel into the Ion AmpliSeq Ready-to-Use or Custom Panel Coverage Analysis plugin data.

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