Torrent Browser User Interface Guide


Torrent Suite Software space on Ion Community

User Interface Guide TOC

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The TMAP documentation pages are in beta release.

TMAP Modules

The Torrent Mapping Alignment Program (TMAP) is a sequence alignment software program optimized specifically for Ion Torrent data. TMAP contains several mapping algorithms, each with its own best application. TMAP's current default is map4 .

When you reanalyze a run, you can optionally change both the TMAP module ( map1 , map2 , map3 , map4 , or mapvsw ) and also change the module's parameters.

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The default TMAP parameters are tuned for Ion data. In most cases, you do not need to modify these settings. Modifying these parameters is recommended for advanced users.

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The main page on reanalyzing a run is Work with Completed Runs .

Mapping modules

This table lists the mapping alternatives supported by TMAP. The map4 module is the default. (Other modules are not run unless specifically called, for instance on the Reanalyze page.)

Click the module name link to see the options supported for that module. See TMAP Global Options for global parameters.

Module Description
map1

BWA-short reads mapping

  • Very fast at finding perfect matches
  • Very slow at finding a set of matches with up to two mismatches
map2 BWA-long / BWASW reads mapping
map3

Simplified SSAHA, based on a k-mer lookup table

map4

Based on the BWA fastmap routine

Searches for the maximum exact matches between the reads and reference

mapvsw

A vectorized implementation of Smith-Waterman

  • A single mapping strategy that is twice as fast as the other modules
  • Modified to improve specificity
mapall A command to quickly map short sequences to a reference genome.

Find the TMAP command for a specific analysis

See Find the TMAP Command for a Completed Run for steps to open the run report log and search for the TMAP command. (The analysis must be completed before you can find the command.)

TMAP examples

This example is the current default setting. Only the map4 module is used.

             tmap mapall -f /results/referenceLibrary/tmap-f3/hg19/hg19.fasta -r /<server_path>/results/analysis/output/Home/Auto_user_G35-685--R65832-110mM_K2SO4-OT_salts-0630_24057_58335/IonXpress_057_rawlib.bam -v -Y -u --prefix-exclude 5 -o 2 stage1 map4
            

This example is the previous TMAP default. This example uses the modules map1 , map2 , and map3 , in that order. Progressively more reads are mapped by each module.

             tmap mapall f <FASTA_file> -v -Y -u --prefix-exclude 5 stage1 map1 map2 map3
            

Access TMAP Frequently Asked Questions on Ion Community: http://ioncommunity.lifetechnologies.com/docs/DOC-9355 .