Torrent Browser User Interface Guide
Torrent Suite Software space on Ion Community
TMAP Modules
The Torrent Mapping Alignment Program (TMAP) is a sequence alignment software program optimized specifically for Ion Torrent data. TMAP contains several mapping algorithms, each with its own best application. TMAP's current default is
map4
.
When you reanalyze a run, you can optionally change both the TMAP module (
map1
,
map2
,
map3
,
map4
, or
mapvsw
) and also change the module's parameters.
Mapping modules
This table lists the mapping alternatives supported by TMAP. The
map4
module is the default. (Other modules are not run unless specifically called, for instance on the Reanalyze page.)
Click the module name link to see the options supported for that module. See TMAP Global Options for global parameters.
Module | Description |
---|---|
map1 |
BWA-short reads mapping
|
map2 | BWA-long / BWASW reads mapping |
map3 |
Simplified SSAHA, based on a k-mer lookup table |
map4 |
Based on the BWA fastmap routine Searches for the maximum exact matches between the reads and reference |
mapvsw |
A vectorized implementation of Smith-Waterman
|
mapall | A command to quickly map short sequences to a reference genome. |
Find the TMAP command for a specific analysis
See Find the TMAP Command for a Completed Run for steps to open the run report log and search for the TMAP command. (The analysis must be completed before you can find the command.)
TMAP examples
This example is the current default setting. Only the
map4
module is used.
tmap mapall -f /results/referenceLibrary/tmap-f3/hg19/hg19.fasta -r /<server_path>/results/analysis/output/Home/Auto_user_G35-685--R65832-110mM_K2SO4-OT_salts-0630_24057_58335/IonXpress_057_rawlib.bam -v -Y -u --prefix-exclude 5 -o 2 stage1 map4
This example is the previous TMAP default. This example uses the modules
map1
,
map2
, and
map3
, in that order. Progressively more reads are mapped by each module.
tmap mapall f <FASTA_file> -v -Y -u --prefix-exclude 5 stage1 map1 map2 map3
Access TMAP Frequently Asked Questions on Ion Community: http://ioncommunity.lifetechnologies.com/docs/DOC-9355 .
Torrent Browser User Interface Guide
The Login Page
The Plan Tab
Templates
Planned Runs
Plan by Sample Set
Create Samples and a Sample Set
Sample Attributes
Template and Planned Run Wizard
Create Multiple Run Plans
Create a Template with Ion AmpliSeq.com Import
The Monitor Tab
The Data Tab
Completed Runs and Reports Tab
Work with Completed Runs
Reanalyze a Completed Run
BaseCaller Parameters
TMAP Parameters
The Projects Listing Page
Project Result Sets Page
Compare Multiple Run Reports
CSV Metrics File Format